There are a variety of SPE stationary phases to choose from including: reverse phase, normal phase, ion-exchange, and mixed mode, with mixed mode being a mixture of both . Grow your laboratory productivity. Reverse Phase. Product Line: SOLA: Sorbent: Mixed Mode Weak Anion Exchanger, Polymeric: Type . Restek. 29 and 30) Common in environmental analysis to reduce leachables from PP hardware and PE frits Reversible SPE Tubes (pg. Rinse with 3 - 5 mL water or buffer. Polymeric MCX SPE cartridges sorbent has a larger surface area compared with other sorbents, so it has a high ion exchange capacity which can keep alkaline material effectively Skip to content +86-29-89284429 Solid-Phase Extraction (SPE) is a sample preparation technique that is used to clean up and/or concentrate samples before analysis. Product Code Product Name Filler Content (mg/cartridge) Package Size; 297-47451: We offer a range of solid phase extraction (SPE) columns and cartridges, packed with a variety of sorbents including regular silica gel 60 and reverse phase silica gels (endcapped and non-endcapped). The stationary phase of a reversed phase SPE cartridge retain compounds of low polarity due to the hydrophobic effect. A novel 2-layer SPE cartridge efficiently reduces background for pesticide determination. This item has been discontinued by the manufacturer and is no longer available. Reversed phase SPE separates analytes based on their polarity. Save Big with Phenomenex's Legendary Savings http://www.phenomenex.com/info/form/17yearendpromoA quick look at how Strata-X polymeric sorbents can simplify t. SPE cartridges are available with many different sorbents. Reversed phase mode refers to the separation mode in which the polarity of the stationary phase of the SPE cartridge is less than the polarity of the mobile phase. Solid Phase Extraction. Reversed-phase SPE cartridges use non-polar adsorbents (carbohydrates or polymers) on the stationary phase to separate organic solutes from the polar phase (water). The C18 solid phase extraction (SPE) cartridge uses octadecyl silica as a filler to retain non-polar compounds by strong hydrophobic interaction. Stable over the pH range (2 - 8). Life sciences & Pharmaceutical: drug discovery, pre-clinical and clinical development, toxicology studies, medical . HLB SPE cartridges. AttractSPEHLB are uncharged water-wettable polymeric resin suitable for the extraction of a wide range of analytes (polar, apolar, neutral, acidic, basic). The following specifications of SPE cartridges are available: Volume: 0.01 ml - 200 ml. Copure QuEChERS . Solid phase extractions use the same type of stationary phases as are used in liquid chromatography columns. Complementary Reversed Phase Retention of Neutral Compounds. Thermo Scientific SOLA SPE Cartridges revolutionize solid phase extraction (SPE). Steroids Extraction By Reversed-Phase Chromatography; SPE Extraction Of Oil & Grease From Water Using SiliaPrep C18 (17%) SPE Extraction Of Phenolic Compounds From Wine Using SiliaPrep C18 (17%) SPE Extraction Of Streptomycin From Food Using SiliaPrep SCX And C18 (17%) We called this reversed phase SPE. The precision and accuracy of the Choosing the best SPE stationary phase to use depends on the physical/chemical properties (such as pH, pKa and Solubility log P) of your analytes. [SPE Column Silica gel-based Normal / Reverse Phase] .We manufacture and distribute chemical reagents for research use only or various antibodies. Solid phase extractions (SPEs) are routinely used in the sample preparation for quantifying analytes in biological fluids such as plasma and urine. Compared with the C18 filler, the C8 filler has a shorter carbon chain and a weaker non-polar hydrophobic interaction. High-purity material with highest reproducibility and lowest blank values due to an optimized manufacturing process. QuEChERS AOAC Kits; QuEChERS EN Kits . Solid phase extraction (SPE) is a sample preparation technique often used by chromatographers prior to analysis. The full range of solid phase extraction (SPE) well plates, columns, disks and accessories from Biotage. . Copure C18N SPE Cartridges 2000mg/12mL 20/Box Write a Comment. Common in environmental Used for large scale purication Reverse SPE tubes prior to elution analysis to reduce leachables (medicinal chemistry purication) to minimize elution volume from PP hardware and PE frits for strongly retained compounds Supel-Tips SPE Rezorian Cartridges Discovery SPE 96-Well Plates Dispersive SPE Resprep C18 SPE Cartridges. Solid Phase Extraction . Please call customer service for assistance: 1-800-766-7000. Copure C18 sorbent is composed of endcapped octadecyl-bounded silica gel particles and retains non-polar compounds by hydrophobic interactions. To perform reversed-phase chromatography with SPE cartridges, use a gradient of strongly to weakly polar solvents [from weak to strong solvent elution strength] with a non-polar packing material.
Available in silica and polymer-based phases for reverse phase, ion exchange, and specific-application needs. Excellent recovery rates, especially for the enrichment of pharmaceuticals and active ingredients, due to the spherical particle shape, homogeneous surface, and optimized pore structure. Details. Product Overview Videos Documents Recommendations Showing 10 of 14 Show more Resprep S-DVB SPE Cartridge High-purity material with highest reproducibility and lowest blank values due to an optimized manufacturing process. Reversed-phase SPE was used in a novel online SPE-HPLC electrochemical and UV detection system for field analysis, with equivalent goals to US EPA Method 8330. An important distinction to keep in mind when employing ion-exchange mechanisms is the nature of the charge state of the analyte. In other words, it may be difficult for a reversed-phase method or bonded chemistry to differentiate between molecules that are structurally similar. Solid Phase Extraction (SPE) Cartridges & Well Plates . In other words, it may be difficult for a reversed-phase method or bonded chemistry to differentiate between molecules that are structurally similar. Copure C18A SPE Cartridges 100mg/1mL 100/Box COC18A3200 . SPE is most often used to remove interfering compounds from a sample, although it can also be used to enrich/concentrate analytes of interest in the sample. Chemistries. Suzuki Catalysts Scavenging Study with Activated Carbon and SiliaMetS; Amine sorbent for the separation of polar compounds; Using Silica-Based Products for Food and Drug Applications; Plastic Chemical Resistance Guide; Reversed-Phase Chromatography: General Introduction for Improved Method Development Thermo Scientific HyperSep C18 Cartridges Get improved retention for most nonpolar compounds and organic analytes in aqueous matrices with these hydrophobic reversed phase C18 cartridges. Thermo Scientific HyperSep C18 Cartridges feature a highly retentive alkyl-bonded phase for nonpolar to moderately polar compounds. However, such blended SPE products do exist, allowing you to utilize ion-exchange and reversed-phase retention mechanisms in the same SPE medium (e.g., polymeric ion-exchange SPE sorbents). Apply sample solution to the top of the packing bed. C18 silica is end-capped. Activated carbon; 50 micron irregular (ISOLUTE-NH2) An important distinction to keep in mind when employing ion-exchange mechanisms is the nature of the charge state of the analyte. Oasis HLB cartridges contain the Oasis HLB sorbent, which is a universal polymeric reversed phase sorbent that was developed for the extraction of a wide range of acidic, basic, and neutral compounds from various matrices using a simple, generic protocol.Chromatography columns separate molecules within mixtures during gas or liquid chromatography. [3] SPE makes use of a solid phase material (there are many to choose from) that . 3 mL, 500 mg. 500 mg/3 mL. Particle Size: 5 m - 250 m. Some of these sorbents are the same types used in HPLC columns. spe columnspe cartridgesolid phase extraction cartridgesolid phase extraction column. . The hydrophobic reversed phase material is retentive for most nonpolar compounds, and retains most organic analytes from aqueous matrices. Always store reversed-phase cartridges in your method's strong solvent (without pH modifiers or . Solid phase extraction (SPE) is a much faster purification method if optimized and it can be done with the standard cartridges as well as 96-well plates, that allow many samples to efficiently be run at the same time. C18, SPE Cartridges, Oro-Sep, 300.0 mg, 100/pkg Reversed phase retention mechanism of the C18 elute allows for the extraction of hydrophobic compounds from aqueous matrices. This is the most commonly used sorbent for sample preparation prior HPLC or GC analysis. The present study reports a precise and simple offline solid-phase extraction (SPE) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of five representative and commonly present pharmaceuticals and personal care products (PPCPs), a new class of emerging pollutants in the aquatic environment. It is particularly suited for the extraction of weak acids (pKa 2 - 8). Highly reproducible recovery rates; cartridge to cartridge, lot to lot. A SPE cartridge consists of a small, open-ended container (often a syringe barrel) filled with particles coated with a sorbent. . Copure C8 sorbent is composed of octyl-bounded silica gel particles and retains non-polar compounds by hydrophobic interactions. Solid-phase extraction (SPE) is a sample preparation method where the compounds that are dissolved or suspended in a liquid mixture are separated from other compounds depending on their physical and chemical properties. Reversed Phase Cartridges. SPE cartridges. Steroids Extraction By Reversed-Phase Chromatography; SPE Extraction Of Oil & Grease From Water Using SiliaPrep C18 (17%) SPE Extraction Of Phenolic Compounds From Wine Using SiliaPrep C18 (17%) SPE Extraction Of Streptomycin From Food Using SiliaPrep SCX And C18 (17%) HPLC, GC, TLC). Solid phase extraction (SPE) is a sample preparation technique often used by chromatographers prior to analysis. Typical applications include . Award-winning fritless polymeric technology eliminates the issues encountered in conventional SPE so that your lab can: . Resprep S-DVB SPE Cartridge. Agilent SampliQ C18 is a reversed-phase sorbent. The first step in using SPE is to select the correct type of adsorbent for the type of separation that is needed.
Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase. These products are available in more than 200 chemistry and format combinations for convenient sample preparation methods based on reverse-phase, normal-phase, ion-exchange, and application-specific specialty chemistries. 24050. Introduction of Three SPE Cartridges' Operation Methods Operation of the reverse phase extraction cartridge Extract non-polar samples from polar matrices 1. The assay involved a solid-phase extraction (SPE) of olanzapine and its internal standard on a Bond Elut Certify LRC mixed-mode cartridge. Description Reversed Phase C18A SPE Cartridges The C18A SPE cartridge uses octadecyl silica as a filler to extract non-polar compounds from an aqueous solution by hydrophobic interaction. . However, such blended SPE products do exist, allowing you to utilize ion-exchange and reversed-phase retention mechanisms in the same SPE medium (e.g., polymeric ion-exchange SPE sorbents). . Description. Normal Phase. C18 Solid Phase Extraction Spe Cartridge , Find Complete Details about C18 Solid Phase Extraction Spe Cartridge,Solid Phase Extraction,Spe Cartridge,200mg Spe Column from Supplier or Manufacturer-Hawach Scientific Co., Ltd. . . Column, well plate, and cartridge formats. Share sensitive information only on official, secure websites. The Agilent SampliQ WAX solid-phase extraction cartridges are compatible with water, acid, basic solvents from pH 0 to 14, and most organic solvents. . The stationary phase of a reversed phase SPE cartridge is derivatized with hydrocarbon chains, which retain compounds of mid to low polarity due to the hydrophobic effect. The solid phase is simply used to "filter" the sample. HyperSep SPE Cartridges and Plates. $112.20 - $339.50 See promotional offers below Specifications View More Specs Products 15 Description Specifications Description Features of Reversed Phase C18 Solid Phase Extraction (SPE) Cartridges CHROMAFIX cartridges offer an alternative way of handling using positive pressure by syringes or peristaltic pumps.. The cartridge . Sample Prep. . Thermo Scientific HyperSep solid phase extraction cartridges offer reproducible, reliable sample preparation in a traditional format. Perform solid phase extraction (SPE) using columns and 96-well plates for sample preparation that improves chromatographic results. Use Thermo Scientific SOLA Solid Phase Extraction (SPE) cartridges and plates to help prevent costly reanalysis of bioanalytical samples by preventing blocking, voiding, and channeling during the SPE sample preparation process. Solid Phase Extraction (SPE) Application Notes. General Extraction Protocols: Reversed Phase Rinse packing bed with 3 - 5 mL methanol. Solid phase extraction (SPE) is a variant of liquid chromatography. 4. High-quality, silica-based hydrophobic adsorbents. Solid-phase extraction (SPE) is a technique designed for rapid sample preparation and purification prior to chromatographic analysis. 24050. . Polar extraction is where the stationary bed is strongly polar in nature and the mobile phase is non-polar or . Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. N/A. Restek. Used to extract hydrophobic analytes from polar matrices, such as water (e.g., pesticides from water). We mainly offer three basic packing types for normal phase, reverse phase and ion exchange extraction as follows: Silica-based C18, C18-ne, C8, C2, PHE, CN, PSA, NH2, SAX, SCX, Silica Polymer-based . Reversed-Phase SPE For extracting non-polar compounds from complex matrices, Biocomma offers several reversed-phase SPE cartridges: most commonly used C18 (endcapped and non-endcapped) and C8. Activation: Rinse the extraction cartridge with 3-5 ml methanol; rinse the extraction cartridge with 3-5 ml deionized water (or buffer) and keep the extraction cartridge moist 2. These correspond to the usual filling materials of HPLC columns, such as reversed phase, normal phase, ion exchange or mixed-mode phase. You can optimize your SPE protocols by using SiliCycle SiliaPrepX Polymeric SPE Cartridges and Well Plates.. The hydrophobic reversed phase material is retentive for most nonpolar compounds, and retains most organic analytes from aqueous matrices. Types. Details. Compared to liquid-liquid extraction, SPE is much faster, uses less solvent, and saves money. 4-Step SPE Procedure. exchange/reversed phased) behavior provides greater flexibility in method design, the ability to fractionate neutral compounds, and cleaner extracts. This is the most commonly used sorbent for sample preparation prior HPLC or GC analysis. The analyte can be eluted by washing the cartridge with a non-polar solvent, which disrupts the interaction of the analyte and the stationary phase. This first fritless SPE product range enables greater sample throughput in bioanalytical laboratories where low elution volumes combined with greater . Reversed phase (C18, C8, cyano, phenyl) Normal phase (silica, diol, NH 2) Ion .
The force between non-polar substances is van der Waals attraction, and the affinity of the agent to the solute mainly depends on its hydrophobicity. Using SiliaPrepX will guarantee the following:. 3 mL, 500 mg. 500 mg/3 mL. 28 Online SPE-HPLC was accomplished using a combination of two six-port valves. Chromatographers use solid phase extraction (SPE) as a sample preparation method-of-choice to improve analytical results by removing interfering compounds, extracting the analyte in a preferred solvent, and concentrating the analyte. Solid Phase Extraction. Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. One clear advantage in SPE-HPLC of explosives, as demonstrated in this and the previous reference, is the . A general protocol can be used to separate weakly basic, weakly acidic, and neutral compounds from complex mixtures. AttractSPE DVB is available under Disk and Powder format as well as under several SPE cartridges size from 1mL to 20mL , reversible formats and for automate. AttractSPESAX is SPE based on strong anion exchange polymeric phase interacting with the analytes via a mixed mode retention mechanism: ion exchange with strong basic functional groups and reversed phase. Reversed Phase SPE . Reversed-Phase SPE Time Hot Copure C18N SPE Cartridges Copure C18A SPE Cartridges Copure C18 SPE Cartridges Copure C8 SPE Cartridges 1/1 Remove unwanted contaminants including phospholipids, proteins, and particulates for superior chromatography Easily cleanup large or small volumes Strata-XL cleans up viscous samples as well as target analytes that exceed 10 kDa The 96-well plate microelution Strata-X formats are ideal for concentrated extraction for small volumes Order Now 4-Step Method. Catalog number: 60109-001. Pore Size: 40 - 450 . Product Category. The hydrophobic reverse phase filler can retain most of the non-polar compounds and most of the organic analytes in the aqueous matrix. In both cases, explosives can be eluted from the SPE cartridge already dissolved in reversed-phase HPLC-compatible solvents, greatly simplifying sample transfer. Push or draw through the bed at 1 - 5 mL/min. SPE provides clean extracts and high recoveries. The analyte is removed from the adsorbent by elution with a suitable solvent. Reversed-Phase SPE Sorbent surface is relatively hydrophobic -Sep-Pak C18 Silica, other alkyl silica, Oasis HLB Sample is prepared for SPE in aqueous or other polar sorbent Oasis HLB is better -pH stable from 1-14 -Doesn't de-wet Examples: Isolation and enrichment of organic contaminants from surface waters