QUALITATIVE PHYTOCHEMICAL SCREENING AND in vitro ANTIMICROBIAL EFFECTS OF METHANOL STEM BARK EXTRACT OF Ficus thonningii (moraceae) *Usman H, Abdulrahman, FI and Usman, A . Screening plants for bioactive agents. Sofowora A. Phytochemical Screening: Screening was carried out on all the Ocimum extracts to determine the active principles in them following the procedures of Sofowora (1993). The extract obtained was accurately weighted and then used for phytochemical screening. Plant Arch, 8 (2) (2008), pp. . In addition to it, Tinospora has been found to exhibit antidiabetic, (Sofowora 1993), hepatoprotective, (Trease and Evans 1989) anticancer, and antioxidant properties as well. An orange colouration indicates the Qualitative phytochemical screening was based on the standard procedures described by Harborne (1973), Trease and Evans (1989), and Sofowora (1993). In vitro antioxidant assay (Osuntokun & Olajubu 2014).
Belandrin, M.F. (1993).Screening plants for bioactive agents, In: Medicinal plants and traditional medicine in Africa. II. (Sofowora, 1993, Okigbo et al. This chapter deals with the qualitative analysis of wild and tissue culture raised regenerants of Artemisia amygdalina, for the amount of bioactive principles particularly the antimalarial compound, artemisinin. (Sofowora, 1993) - - - 23 . The screening involves detection of alkaloids, flavonoids, terpenoids, saponins, tannins, anthraquinones, and cardiac glycosides. 21. - References - Scientific Research Publishing Article citations More>> Sofowora, A. Harbone JB (1998) Phytochemical Methods, Chapman and Halls: London 91. . The phytochemical screening was carried out using standard procedure (Sofowora, 1993 and Trease and Evans, 2002). reducing sugars. A. ringens.
Phytochemical Screening Phytochemical screening of the extract was carried out to identify the constituents, using standard phytochemical methods as described by Trease and Evans (1989) as well as Sofowora (1993). Google Scholar. These results confirm the use of this plant in traditional medicine for the treatment of bacterial infections and diseases associated to oxidative stress such as diabetes. The phytochemical screening for major constituents was undertaken using standard qualitative methods as described by Trease and Evans (1989), Harbone (1985), Odebiyi and Sofowora (1990) and Sofowora (1993). The current study was aimed at investigating the antipyretic, anti-inflammatory and analgesic activity of methanol extract of Terpenes play a very Roof Ul Qadir Scholars who have joined in 2019 for Integrated Ph A study was conducted to assess the relation between intake of phytochemicals rich foods and obesity by calculating the phytochemical index Phytochemical . Phytochemical screening of Jatropha tanjorensis leaf revealed that it contains . Proximate and heavy metal analysis The proximate composition and light metal analysis were carried out using the methods described by Ikhuoria et al., (2008). Alkaloid determination 2.5 g of the powder was extracted using 100 ml of 20% acetic acid in ethanol. A phytochemical screening of the extracts was also carried out to determine the constituents of garlic. use as reported (Sofowora 1993). The phytochemical screening of the aqueous and alcoholic extracts of the leaf of A. indica was (Sofowora, 1993). The solution was filtered through a vacuum pump. . Quantitative Determination of Phytochemicals201 Determination of Tannin: The tannin content of the Phytochemical Screening . Debiyi OO, Sofowora FA. Standard methods were used for the . Anticancer Potency of Seagrass (Enhalus acoroides) Methanol. 20. 657-662. (Sofowora, 1993). Phytochemical screening of Jatropha tanjorensis leaf revealed that it contains . Preliminary Phytochemical screening was performed for characterization of different bioactive compounds present in S. urens Roxb.. Alkaloids (Mayer's test): 6 ml of extract was mixed with 6 ml 1% . Flavonoids are known to have antioxidant effects and have been shown to inhibit the initiation, promotion and progression of tumors (Kim et al., 1994). Spectrum Books Ltd., Nigeria, 150-156. Agar well diffusion method was employed for the antimicrobial screening of the extracts of the leaves of Tectona grandis and its mistletoe. Sofowora, 1993 used for the SaponinsFoam test in 3 mL of dist. Test for flavonoids: To 1 mL of methanolic extract, few drops of conc.
ABSTRACT: The phytochemical and nutritional composition of Trichosanthes cucumerina L. an underutilized vegetable used as substitute for Solanaceous tomatoes by rural dwellers was examined in comparison with majorly cultivated tomato cultivars in Nigeria (Roma VF and Ibadan local). 3.3.2 Test . Methods for Phytochemical Screening Phytochemical screening was performed using standard procedures (Sofowora, 1993, Trease and Evans, 1989, Ayoola et al.,2008). Test for alkaloids 100 mg of powdered sample was dissolved in 5 ml of methanol and then filtered. (Edeoga et al. Azadirachta indica Juss (neem) is a plant which has been used for a long time as traditional medicine for household remedy against various human ailments from (1993) Phytochemical Screening of Medicinal Plants and Traditional Medicine in Africa Edition. a. b. temperature following the method suggested by Sofowora (1993). (Osuntokun & Olajubu 2014). Vahl. University of Ife Press 2nd Ed. Phytochemical screening of Senna occidentalis leaves The phytochemical screening was was . Search: Phytochemical Ppt. Phytochemical screening of extracts revealed the. immunomodulatory activities. Sofowora, 1993) (Odebiyi and Sofowora, 1978; Trease and Evans, 1989). Aristolochia. BEST 3: 162-164. Phytochemical Screening Phytochemical screening of the extract was carried out to identify the constituents, using standard phytochemical methods as described by Trease and Evans (1989) as well as Sofowora (1993). Standard methods as outlined by Sofowora (Reference Sofowora 1993) were employed in phytochemical screening tests . Estimation of antioxidant activity . Phytochemical Screening Chemical tests were carried out ethanolic fruit and leaves extracts for the qualitative determination of phytochemical constituents as describe by Harborne (1973), Trease and Evans (1989), Sofowora (1993) as cited by Abbhi, Joseph and George (2011). A change in colour from pink to violet showed the presence of terpenoids ( Sofowora, 1993 ). Qualitative Method of Analyses Plant filtrate were prepared by boiling 20 g of the fresh plant in distilled water. Every year, the Phytochemical Society of Europe makes available a number of travel bursaries to assist young researchers to attend PSE-sponsored conferences Though most studies have focused on the better-known green and black teas, white and oolong also bring benefits to the table Flavonoids belong to the polyphenol family The study on antioxidant activity of ulvan . Qualitative Phytochemical Analysis: Chemical tests were carried out on the aqueous extract using modified standard procedures to identify the constituents as described by Sofowora(1993), Trease and Evans(1989) and Harborne(1973). The presence of these phytochemicals in the sap of Musa paradisiaca confers medicinal properties on the plant and this explains the Sofowora A. Phytochemical Screening of Medicinal Plants and Traditional Medicine in Africa, Nigeria: Spectrum Books Ltd; 1993.
Steroids were highly detected in all extracts with the exception of the ethanolic seed extract. The phytochemical tests was carried out using standard methods of analysis and these investigations revealed the presence of alkaloids, anthraquinones, carbohydrates, flavonoids, saponins and tannins. Sofowora (1993). Sofowora A., (1993). Determination of antioxidant activity Rapid-TLC screening for antioxidant activity was carried out by spotting a concentrated methanolic solution of the extract on silica gel plates. One milliliter of mixture was taken separately in two test tubes. Flavonoids are known to have antioxidant effects and have been shown to inhibit the initiation, promotion and progression of tumors (Kim et al., 1994).
Qualitative Phytochemical analysis The Qualitative analysis of the extracts was carried out in accordance with standard methods as described by Harborne (1973), Trease and Evans (1989) and Sofowora (1993). According to a research by World Health Organisation in 2001 an estimated 80% of the population of developing countries in Africa and Asia are known to rely on traditional medicine, mostly plant drugs for their primary health care needs. (1993). The use of plant extracts in traditional medicine is a worldwide practice.
The Phytochemical screening of seagrass extract included tannins, flavonoids, alkaloids, saponins, and terpenoids test [9, 10]. Quantitative screening 2.3.2.1.
Phytochemical Screening Test The phytochemical constituents of the plant were detected using standard procedures as described by Trease and Evans (2002) [10] and Sofowora, (1993) [11]. N . The filtrate was heated with 5 ml of . Stock solutions of each extract with a concentration of 10 mg extract/mL distilled water was prepared and used for the phytochemical screening. H 2 O it gives the foam in the methanol extract and no or less foam to the water extract 6] Sofowora, 1993 gives the test for the . Phytochemical screening showed very strong presence of Alkaloids, Glycosides, Flavonoids, Reducing compounds and Polyphenol in both aqueous and ethanol extract of the leaves while the bark showed appreciable presence of Alkaloids, Reducing compounds and Polyphenol in both aqueous and ethanol extracts. The screening involves detection of alkaloids, flavonoids, terpenoids, saponins, tannins, anthraquinones, and cardiac glycosides. . and S.oblonga were assessed by the standard method as described by Sofowora (1993) [9], . Sofowora A (1999) African Medicinal Plants. Sofowora AE. Phytochemical screening of Dracaena arborea (Asparagaceae) for insecticidal activity in the control of Sitophilus zeamais (Coleoptera: Curculionidae) and Callosobruchus maculatus (Coleoptera: Chrysomelidae) - Volume 33 Issue 2 . Sofowora A (1993) Medicinal plants and traditional medicine in Africa . The . According to a research by World Health Organisation in 2001 an estimated 80% of the population of developing countries in Africa and Asia are known to rely on traditional medicine, mostly plant drugs for their primary health care needs. Quantitative Determination of Phytochemicals201 Determination of Tannin: The tannin content of the The screening involves detection of alkaloids, flavonoids, terpenoids, saponins, tannins, anthraquinones, and cardiac glycosides. Qualitative Method of Analyses Plant filtrate were prepared by boiling 20 g of the fresh plant in distilled water.
The study aimed at ascertaining the phytochemical constituents, antimicrobial and cytotoxic activities of chloroform extracts of. Test for glycosides One millilitre (1 ml) of the leaf extract was dissolved in 1ml of glacial acetic acid containing one drop of ferric chloride solution. 15th . The filtrate were used for the phytochemical screening for flavonoids, tannins, saponins, alkaloids, reducing sugars, Phytochemical Screening and Antibacterial Assay of Radish Seed Oil on Selected Pathogenic Bacterial Species in Vitro. American Journal of Biomedical and Life Sciences . Qualitative Phytochemical Analysis: Chemical tests were carried out on the aqueous extract using modified standard procedures to identify the constituents as described by Sofowora(1993), Trease and Evans(1989) and Harborne(1973). Phytochemical screening of the extract was carried out to identify the constituents, using standard phytochemical methods as described by Trease and Evans (1989) as well as Sofowora (1993)..
Sofowora, A.O. Phytochemical screening and mineral composition of . 1978;3:234-46. (1993). Preliminary phytochemical screening Qualitative analysis of the crude extracts was carried out as described previously (Brain and Turner, 1975; Sofowora, 1993; Edeoga et al. Phytochemical screening of medical plants.
Phytochemical screening revealed the presence of alkaloids and steroids in all the extracts. Sofowora (1993). Phytochemical Screening Test The phytochemical constituents of the plant were detected using standard procedures as described by Trease and Evans (2002) [10] and Sofowora, (1993) [11]. The filtrate were used for the phytochemical screening for flavonoids, tannins, saponins, alkaloids, reducing sugars, Test for tannin: About 0.5 g of the dried powdered samples were boiled in 20 ml of water in a test-tube and then filtered, a few drops of 0.1% ferric chloride was added and observed for brownish green or a blue black . Search: Phytochemical Ppt. The acute toxicity of the N. nucifera fruit extract in mice revealed its LD50 value to be greater than 5000 mg/kg. Fehling's test for free reducing sugar: About 0.5 g each portion was dissolved in distilled water and filtered. Triterpenoids. Quantitative analysis of phytochemical constituents Phytochemical screening of Nigerian Medicinal Plants Part III,Lloyida 1990; Pp . . 2.4.2. Spectrum Books ltd (Ibadan) 142-144. screened for the presence of phytochemical constituents such as alkaloids, terpenoids, anthraquinones, flavonoids, tannins, saponins, steroids and glycosides using qualitative phytochemical screening tests described by Sofowora (1993) and Trease and Evans (1989).
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